- Captain Kirk
 - Biography of the character Captain James Kirk, in command of the Federation starship U.S.S. Enterprise, and voiced by William Shatner in the animated Star Trek series. - Dr. McCoy
- Profile of the fictional, futuristic Doctor Leonard "Bones" McCoy, voiced by DeForest Kelley on Star Trek: The Animated Series.
- Lt. Arex
- Profile of the Star Trek: The Animated Series navigator, voiced by James Doohan and replacing Ensign Chekov.
- Lt. M'Ress
- Profile of the Star Trek: The Animated Series junior engineer, who was voiced by Majel Barrett Roddenberry.
- Lt. Sulu
- Profile of the helmsman and weapons officer on the U.S.S. Enterprise, voiced by George Takei in Star Trek: The Animated Series.
- Lt. Uhura
- Biography of Lieutenant Uhura, communications officer on the U.S.S. Enterprise, voiced by Nichelle Nichols on Star Trek: The Animated Series.
- Mr. Spock
- Biography of the half-human, extremely logical first officer of the U.S.S. Enterprise, voiced by Leonard Nimoy in the Star Trek animated series.
- Nurse Chapel
- Biography of the Starfleet Lieutenant and Head Nurse of the U.S.S. Enterprise, voiced by Majel Barrett Roddenberry on the Star Trek animated series.
- Scotty
- Biography of fiery Scottish engineer Montgomery Scott, voiced by James Doohan on Star Trek: The Animated Series.
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