- American Compliance Institute (ACI)
 - Not-for-profit, national membership organisation which offers education, training and research in corporate compliance issues. - Animal Welfare Institute (AWI)
- Nonprofit charitable organisation dedicated to reducing cruelty towards animals.
- Blackwater Creek Association
- Promotes the cultural, architectural, historical, and natural heritage of the environs of Blackwater Creek, Upper and Lower Canal Basins, and James River.
- Catoctin Conservation District
- Seeking to protect and preserve the rural landscape in the Catoctin Creek scenic river area, through perpetual open space easements and acquisition.
- Charles Darwin Foundation, Inc.
- Dedicated to promoting conservation, education, and scientific research in and on behalf of the Galapagos Islands.
- Clean Fairfax Council
- Educates citizens on litter prevention, litter control, supports the county recycling programme, discourages graffiti.
- Conservation Fund, The
- Dedicated to land and water conservation.
- Mattaponi and Pamunkey Rivers Association
- Dedicated to the history, ecology, scenic landscape, recreation, and economy of the Mattaponi and Pamunkey rivers.
- National Capital Dahlia Society
- Promotes growing dahlias with an emphasis upon growers in the metro D.C. area. Includes calendar, member resources, and information on the Mid-Atlantic Trial Garden.
- National Recycling Coalition@
- National Water Resources Association
- Advocates Federal policies, legislation and regulations promoting the development, management, protection and beneficial use of water resources.
- New River Land Trust
- Works with landowners and local governments in southwest Virginia to protect land for farming, forestry, natural habitat, and recreation.
- Oceanwatch
- Working to conserve oceans, marine life, and coral reefs through education and grassroots action.
- Piedmont Environmental Council
- Supporting the environment in the Piedmont region of Virginia.
- Public Lands Foundation
- Dedicated to the proper use, protection, and professional management of federal lands administered by the Bureau of Land Management.
- Roanoke Valley Greenways
- Offers information about the Roanoke Valley of Virginia's network of greenways - corridors of protected open space managed for conservation, recreation, and non-motorized transportation.
- Science & Environmental Policy Project
- A nonprofit, non-partisan public policy institute dedicated to making sound science the basis for environmental policy decisions.
- Smith Mountain Lake Association
- Protecting the lake.
- Valley Conservation Council
- Promotes land use that sustains the farms, forests, open spaces, and cultural heritage of the Valley of Virginia.
- York Watershed Council
- York River Basin's seven SWCDs take advantage of the opportunity that the Chesapeake Bay Tributary Nutrient Reduction Strategy process represents.
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